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inverted fluorescent microscope nikon eclipse te2000-u  (Nikon)


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    Structured Review

    Nikon inverted fluorescent microscope nikon eclipse te2000-u
    Inverted Fluorescent Microscope Nikon Eclipse Te2000 U, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te2000-u+fluorescence+microscope/pm40250144-42-14-16
    Average 90 stars, based on 1 article reviews
    inverted fluorescent microscope nikon eclipse te2000-u - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Roles of cMyBP-C phosphorylation on cardiac contractile dysfunction in db/db mice
    Article Snippet: Perfusion was then switched to the same solution containing Liberase (0.25 mg/ml, Roche) until the heart became flaccid (~8–10 min). .. Isolated cardiomyocytes were incubated at room temperature for 15 min in the media containing Ca 2+ -sensitive Fura-2 dye at a final concentration of 1.0 μM (Invitrogen) and subsequently washed with Tyrode’s solution twice for 10 min. After that, live cells were placed in a perfusion chamber on the stage of an inverted Nikon eclipse TE2000-U fluorescence microscope. .. Using a video-based sarcomere length detection system (IonOptix, Milton, MA, USA), sarcomere shortening was measured.

    Incubation:

    Article Title: Roles of cMyBP-C phosphorylation on cardiac contractile dysfunction in db/db mice
    Article Snippet: Perfusion was then switched to the same solution containing Liberase (0.25 mg/ml, Roche) until the heart became flaccid (~8–10 min). .. Isolated cardiomyocytes were incubated at room temperature for 15 min in the media containing Ca 2+ -sensitive Fura-2 dye at a final concentration of 1.0 μM (Invitrogen) and subsequently washed with Tyrode’s solution twice for 10 min. After that, live cells were placed in a perfusion chamber on the stage of an inverted Nikon eclipse TE2000-U fluorescence microscope. .. Using a video-based sarcomere length detection system (IonOptix, Milton, MA, USA), sarcomere shortening was measured.

    Article Title: Von Willebrand Factor Synergizes with Tumor-Derived Extracellular Vesicles to Promote Gastric Cancer Metastasis
    Article Snippet: They were incubated with gcEVs or VWF + gcEVs, with or without the presence of the VWF-blocking antibody (Abcam). .. After washing, the cells were incubated with 5×10 /well of PKH26 + AGS or HGC-27 cells for 24 hrs at 37°C and the PKH26 + cells that migrated to the opposite side of the membrane were counted under a Nikon Eclipse TE2000-U fluorescence microscope. ..

    Concentration Assay:

    Article Title: Roles of cMyBP-C phosphorylation on cardiac contractile dysfunction in db/db mice
    Article Snippet: Perfusion was then switched to the same solution containing Liberase (0.25 mg/ml, Roche) until the heart became flaccid (~8–10 min). .. Isolated cardiomyocytes were incubated at room temperature for 15 min in the media containing Ca 2+ -sensitive Fura-2 dye at a final concentration of 1.0 μM (Invitrogen) and subsequently washed with Tyrode’s solution twice for 10 min. After that, live cells were placed in a perfusion chamber on the stage of an inverted Nikon eclipse TE2000-U fluorescence microscope. .. Using a video-based sarcomere length detection system (IonOptix, Milton, MA, USA), sarcomere shortening was measured.

    Fluorescence:

    Article Title: Roles of cMyBP-C phosphorylation on cardiac contractile dysfunction in db/db mice
    Article Snippet: Perfusion was then switched to the same solution containing Liberase (0.25 mg/ml, Roche) until the heart became flaccid (~8–10 min). .. Isolated cardiomyocytes were incubated at room temperature for 15 min in the media containing Ca 2+ -sensitive Fura-2 dye at a final concentration of 1.0 μM (Invitrogen) and subsequently washed with Tyrode’s solution twice for 10 min. After that, live cells were placed in a perfusion chamber on the stage of an inverted Nikon eclipse TE2000-U fluorescence microscope. .. Using a video-based sarcomere length detection system (IonOptix, Milton, MA, USA), sarcomere shortening was measured.

    Article Title: Selective neuronal knockout of STAT3 function inhibits epilepsy progression, improves cognition, and restores dysregulated gene networks in a temporal lobe epilepsy model
    Article Snippet: .. Images of ZnT3 staining in the inner molecular layer (IML) above the upper blade of DGCs and ipsilateral to IHSaline or IHKA injection were obtained with a Nikon Eclipse TE2000-U fluorescence microscope at 10X magnification ( G ). ..

    Article Title: Ruxolitinib-dependent reduction of seizure load and duration is accompanied by spatial memory improvement in the rat pilocarpine model of temporal lobe epilepsy.
    Article Snippet: Molecules with optimized pharmacokinetic properties selectively aimed at the inhibition of STAT3 phosphorylation in brain have recently emerged as potential disease modifying therapies for epilepsy.. In the current study, pharmacological inhibition of JAK1/2 with the orally available, FDA-approved drug ruxolitinib, produced nearly complete inhibition of hippocampal STAT3 phosphorylation, and reduced the expression of its downstream target Cyclin D1, when administered to rats 30 min and 3 h after onset of pilocarpine-induced status epilepticus (SE).. This effect was accompanied by significantly shorter seizure duration and lower overall seizure frequency throughout the 4 weeks of EEG recording, but did not completely prevent the development of epilepsy in ruxolitinib-treated male rats.

    Article Title: Benzophenone-3 alters expression of genes encoding vascularization and epithelial-mesenchymal transition functions during Trp53-null mammary tumorigenesis.
    Article Snippet: Benzophenone-3 (also referred to as oxybenzone) is a putative endocrine disrupting chemical and common ingredient in sunscreens and other personal care products.. We previously showed that benzophenone-3 was promotional for epithelial tumorigenesis in mice fed adult high-fat diet, while protective against the incidence of more aggressive spindle cell tumors in the same treatment group.. In this study, we show that benzophenone-3 reduces epithelial to mesenchymal transition in the epithelial tumors of these mice.

    Article Title: Von Willebrand Factor Synergizes with Tumor-Derived Extracellular Vesicles to Promote Gastric Cancer Metastasis
    Article Snippet: They were incubated with gcEVs or VWF + gcEVs, with or without the presence of the VWF-blocking antibody (Abcam). .. After washing, the cells were incubated with 5×10 /well of PKH26 + AGS or HGC-27 cells for 24 hrs at 37°C and the PKH26 + cells that migrated to the opposite side of the membrane were counted under a Nikon Eclipse TE2000-U fluorescence microscope. ..

    Microscopy:

    Article Title: Roles of cMyBP-C phosphorylation on cardiac contractile dysfunction in db/db mice
    Article Snippet: Perfusion was then switched to the same solution containing Liberase (0.25 mg/ml, Roche) until the heart became flaccid (~8–10 min). .. Isolated cardiomyocytes were incubated at room temperature for 15 min in the media containing Ca 2+ -sensitive Fura-2 dye at a final concentration of 1.0 μM (Invitrogen) and subsequently washed with Tyrode’s solution twice for 10 min. After that, live cells were placed in a perfusion chamber on the stage of an inverted Nikon eclipse TE2000-U fluorescence microscope. .. Using a video-based sarcomere length detection system (IonOptix, Milton, MA, USA), sarcomere shortening was measured.

    Article Title: Selective neuronal knockout of STAT3 function inhibits epilepsy progression, improves cognition, and restores dysregulated gene networks in a temporal lobe epilepsy model
    Article Snippet: .. Images of ZnT3 staining in the inner molecular layer (IML) above the upper blade of DGCs and ipsilateral to IHSaline or IHKA injection were obtained with a Nikon Eclipse TE2000-U fluorescence microscope at 10X magnification ( G ). ..

    Article Title: Ruxolitinib-dependent reduction of seizure load and duration is accompanied by spatial memory improvement in the rat pilocarpine model of temporal lobe epilepsy.
    Article Snippet: Molecules with optimized pharmacokinetic properties selectively aimed at the inhibition of STAT3 phosphorylation in brain have recently emerged as potential disease modifying therapies for epilepsy.. In the current study, pharmacological inhibition of JAK1/2 with the orally available, FDA-approved drug ruxolitinib, produced nearly complete inhibition of hippocampal STAT3 phosphorylation, and reduced the expression of its downstream target Cyclin D1, when administered to rats 30 min and 3 h after onset of pilocarpine-induced status epilepticus (SE).. This effect was accompanied by significantly shorter seizure duration and lower overall seizure frequency throughout the 4 weeks of EEG recording, but did not completely prevent the development of epilepsy in ruxolitinib-treated male rats.

    Article Title: Benzophenone-3 alters expression of genes encoding vascularization and epithelial-mesenchymal transition functions during Trp53-null mammary tumorigenesis.
    Article Snippet: Benzophenone-3 (also referred to as oxybenzone) is a putative endocrine disrupting chemical and common ingredient in sunscreens and other personal care products.. We previously showed that benzophenone-3 was promotional for epithelial tumorigenesis in mice fed adult high-fat diet, while protective against the incidence of more aggressive spindle cell tumors in the same treatment group.. In this study, we show that benzophenone-3 reduces epithelial to mesenchymal transition in the epithelial tumors of these mice.

    Article Title: Von Willebrand Factor Synergizes with Tumor-Derived Extracellular Vesicles to Promote Gastric Cancer Metastasis
    Article Snippet: They were incubated with gcEVs or VWF + gcEVs, with or without the presence of the VWF-blocking antibody (Abcam). .. After washing, the cells were incubated with 5×10 /well of PKH26 + AGS or HGC-27 cells for 24 hrs at 37°C and the PKH26 + cells that migrated to the opposite side of the membrane were counted under a Nikon Eclipse TE2000-U fluorescence microscope. ..

    Staining:

    Article Title: Selective neuronal knockout of STAT3 function inhibits epilepsy progression, improves cognition, and restores dysregulated gene networks in a temporal lobe epilepsy model
    Article Snippet: .. Images of ZnT3 staining in the inner molecular layer (IML) above the upper blade of DGCs and ipsilateral to IHSaline or IHKA injection were obtained with a Nikon Eclipse TE2000-U fluorescence microscope at 10X magnification ( G ). ..

    Injection:

    Article Title: Selective neuronal knockout of STAT3 function inhibits epilepsy progression, improves cognition, and restores dysregulated gene networks in a temporal lobe epilepsy model
    Article Snippet: .. Images of ZnT3 staining in the inner molecular layer (IML) above the upper blade of DGCs and ipsilateral to IHSaline or IHKA injection were obtained with a Nikon Eclipse TE2000-U fluorescence microscope at 10X magnification ( G ). ..

    other:

    Article Title: Borna disease virus 2 maintains genomic polymorphisms by superinfection in persistently infected cells
    Article Snippet: HEK293T cells seeded into 6-well plates were transfected with 2.0 μg of BoDV-2 cDNA plasmid, 0.5 μg of pCAGGSBoDV-2 N, 0.025 μg of pCAGGS-BoDV-2 P, 0.25 μg of pCAGGS-BoDV-1 L, 0.04 μg of pCAGGS-BoDV-2 M, and 0.01 μg of pCAGGS-BoDV-2 G using TransIT293 according to the manufacturer’s instructions.

    Membrane:

    Article Title: Von Willebrand Factor Synergizes with Tumor-Derived Extracellular Vesicles to Promote Gastric Cancer Metastasis
    Article Snippet: They were incubated with gcEVs or VWF + gcEVs, with or without the presence of the VWF-blocking antibody (Abcam). .. After washing, the cells were incubated with 5×10 /well of PKH26 + AGS or HGC-27 cells for 24 hrs at 37°C and the PKH26 + cells that migrated to the opposite side of the membrane were counted under a Nikon Eclipse TE2000-U fluorescence microscope. ..



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